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Detecting Two Organisms in One Step
DKA extends the power of the GEN-PROBE Hybridization Protection Assay (HPA) technology by allowing the simultaneous detection of amplicon from two independent amplification reactions (multiplex amplification).
Two different acridinium ester molecules are attached to two different DNA probes. One of the acridinium ester molecules has fast light-off kinetics and is called a "flasher." The other acridinium molecule has slower kinetics and is called a "glower". By targeting two different target sequences, assays can be developed to simultaneously detect two different organisms at a time. Thus, two results can be obtained simultaneously in the same test tube, using one specimen in one reaction. No reflex testing is necessary to identify the organism.
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Gen-Probe Incorporated
10210 Genetic Center Drive
San Diego, CA 92121
USA
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