Elucigene QST*R products are a range of DNA based, QF-PCR, multiplexed diagnostic assays that use STR markers to detect the three most common viable autosomal trisomies: trisomy 21 (Down syndrome), trisomy 18 (Edwards syndrome) and trisomy 13 (Patau syndrome). Additional markers on the sex chromosomes X and Y can detect the most common sex chromosome aneuploidies, Klinefelter syndrome and Turner syndrome.

Using QF-PCR, the QST*R series of rapid aneuploidy testing kits — fully validated for in vitro diagnostic use and suitable for both chorionic villus and amniotic fluid analysis — makes the powerful QF-PCR technique widely available for routine use in molecular cytogenetic procedures.

Elucigene QST*R rapid aneuploidy QF-PCR test provides:

• a simple one tube, highly multiplexed, QF-PCR assay - minimal transfer steps
• reagents ready mixed - just add DNA
• markers informative in more than 99% of analyses
• a rapid aneuploidy test - can provide a definitive result within 4 hours, including DNA extraction
• assays validated for use on Applied Biosystems' 3*** Genetic Analyzers
• a test developed in collaboration with Guy's and St Thomas' Hospital, London

The method employed by Elucigene QST*R kits is the QF-PCR (Quantitative Fluorescence-Polymerase Chain Reaction) technique. Using PCR amplification, fluorescent dye labelled primers target highly polymorphic regions of DNA sequence, short tandem repeats (STRs), that are located on the chromosomes of interest. Each targeted STR marker is specific to the chromosome on which it is located, thus the copy number of the STR marker can be diagnostic of the copy number of the chromosome.

A normal sample has the normal complement of two each of the autosomal chromosomes and two sex chromosomes, thus two alleles of a chromosome specific STR are determined by the QF-PCR technique as two peaks in a 1:1 ratio. The QF-PCR observation of an extra STR allele as either a three peak pattern in a 1:1:1 ratio or two peak pattern in a 2:1 or a 1:2 peak ratio is diagnostic of the presence of an additional sequence which in turn may represent an additional chromosome, as in the case of a trisomy.

This product is not approved by the FDA and is not available for sale in the US

The neccessary analysis files and settings for GeneMarker and GeneMapper are provided below:

GeneMapper 3.0 and above

GeneMapper settings

Plot Settings (xml) Analysis Settings (xml) Table Settings (xml) Size Standard Settings (xml) Bin File (txt) Panel File (txt)

GeneMapper Report Templates

QST*R (xls) QST*Rplusv2 (xls) QST*R-XYv2 (xls) QST*R-13 (xls) QST*R-18 (xls) QST*R-21 (xls)

GeneMarker

GeneMarker Panel Files

QST*Rplusv2 QST*R-XYv2 QST*R QST*R-13 QST*R-18 QST*R-21

Size Standard Files

LIZ500 size ladder LIZ600 size ladder

GENEMAPPER is a trademark of Applied Biosystems, LLC.

GENEMARKER is a trademark of SoftGenetics.

All QST*R kits comply with the IVDD EC Directive 98/79/EC, carry the CE mark and are manufactured within quality systems accredited to ISO9001:2008 and ISO13485:2003. Full technical support is provided to purchasers of kits to ensure acceptable performance and customer satisfaction.

This product is not approved by the FDA and is not available for sale in the US.

For additional availability in other countries please contact your local sales rep or distributor.

For information on our Elucigene QST*R range please download our brochures:

A4 brochure: Click to Download